A TVA-single-chain antibody fusion protein mediates specific targeting of a subgroup A avian leukosis virus vector to cells expressing a tumor-specific form of epidermal growth factor receptor.

Epidermal Growth Factor Receptor Cells Expressing a Tumor-Specific Form of Subgroup A Avian Leukosis Virus Vector to Protein Mediates Specific Targeting of a Single-Chain Antibody Fusion

Targeting avian leukosis virus subgroup A vectors by using a TVA-VEGF bridge protein.

Previously, we have demonstrated that bridge proteins comprised of avian leukosis virus (ALV) receptors fused to epidermal growth factor (EGF) can be used to selectively target retroviral vectors with ALV envelope proteins to cells expressing EGF receptors. To determine whether another type of ligand incorporated into an ALV receptor-containing bridge protein can also function to target retrovi...

Metastatic cutaneous neuro- myofibroblastic sarcoma induced by avian leukosis virus subgroup J in a rooster (Gallus gallus domesticus)

An adult native cock (Gallus gallus domesticus) referred to the aviary clinic with multiple different sizes of round dermal nodules. The bird died few days later, and was then submitted for further evaluation. Macroscopic and microscopic examinations as well as a PCR test were done to identify type and cause of the tumor. In histopathological assessment of biopsy specimen, it consisted of inter...

Novel monoclonal antibody directed at the receptor binding site on the avian sarcoma and leukosis virus Env complex.

We report here on the generation of a mouse monoclonal antibody directed against Rous sarcoma virus (RSV) subgroup A Env that will be useful in functional and structural analysis of RSV Env, as well as in approaches employing the RCAS/Tva system for gene targeting. BALB/c mice were primed and given boosters twice with EnvA-expressing NIH 3T3 cells. Resulting hybridomas were tested by enzyme-lin...

Design and Construction of a Novel Humanized Single-Chain Variable-Fragment Antibody against the Tumor Necrosis Factor alpha

The pro-inflammatory cytokine, TNF-α, which plays a major role in the development and persistence of inflammatory diseases, is the basis for the use of anti-TNF-α therapies. The neutralization of TNF-α or blockage of its binding to the corresponding receptor has mainly served as a therapeutic strategy against some diseases. This study aimed to investigate the production of a humanized single ch...